All stratified-random sampling begins no sooner than an hour prior to sunset and continues into the night. Soak times are kept to approximately 30 minutes to standardize effort and ensure red drum are tagged in good condition. All random samples are conducted using a 1,500-meter bottom longline, with 100 hooks per set, that is retrieved using a hydraulic reel. The hooks are baited with cut striped mullet, but in the event mullet is not available squid is used. Catch per unit effort (CPUE) is calculated as the number of red drum captured per 100 hooks. Environmental and habitat data is recorded during the haul back of each main line and includes location, duration/soak time, gear parameters, sediment size, bottom composition, depth (m), surface and bottom temperature (°C), surface and bottom salinity (ppt), surface and bottom dissolved oxygen (mg/L), weather description, wind direction, wind speed (knots), and location (lat-longs in degrees, minutes seconds) of tagged and released fish.
All red drum captured are measured (FL and TL) to the nearest millimeter and the presence or absence of ‘drumming’, indicative of males only, is noted. A fin clip taken from the caudal fin is removed for genetic analysis. Red drum that will be released are tagged using either a stainless-steel dart tag (>550 mm FL) or an internal anchor tag (<550 mm FL). In addition, all released red drum are implanted with a Passive Integrated Transponder (PIT) tag in the musculature just below the junction of the first and second dorsal fins. Double tagging red drum allows long-term tag retention rates to be determined. Additionally, the PIT tags allow recaptures in the survey to be documented, even if the external tag is not present. A systematic random subsample of red drum is retained for age (otoliths), sex determination, maturity information, genetic data, and diet analysis.
In addition to red drum, other finfish and shark species captured in the survey are enumerated and measured with pertinent biological data recorded. Coastal shark species that are in good condition are tagged as part of the National Marine Fisheries Service Cooperative Shark Tagging Program.